Cloning and Purification of the Hydrophilic Fragment of Hepatitis C Virus E2 Protein Fused to the Choline-binding Domain of the Major Autolysin of Streptococcus pneumoniae: Evaluation of the Humoral Immune Response in Rabbits
نویسندگان
چکیده
The hydrophilic fragment of hepatitis C virus (HCV) E2 protein was expressed in Escherichia coli as a chimera, bound to the choline-binding domain (C-Lyt) of the major autolysin of Streptococcus pneumoniae. The C-LytE2 chimera was purified by affinity chromatography using DEAE-Sepharose. The purified antigen was used to immunize rabbits and the specific humoral immune response to E2 protein was examined. Induction to high levels of antibodies against this HCV E2 protein was found.
منابع مشابه
Cloning of Minor Autolysin of Streptococcus Pneumoniae
Abstract Background and Objective: Increased antibiotic resistant strains and inadequacy of current vaccines against pneumococcal infections necessitate the study of novel protein antigens. It seems that minor autolysin of Streptococcus pneumoniae may have antigenicity. Thus, we aimed at cloning its gene for the first time. Material and Methods: After DNA extraction of Streptococcus pneumoniae ...
متن کاملCloning and expression of fragment of the rabies virus nucleoprotein gene in Escherichia coli and evaluation of antigenicity of the expression product
Rabies virus nucleoprotein (N protein) encapsidates genomic RNA of the virus and forms the viral ribonucleoprotein complex. These N proteins represent highly organized structures which activate proliferation of B cells and production antibodies against the N protein. In addition to the B cell, the rabies virus N protein has been shown to induce potent T helper cell responses resulting in a long...
متن کاملConstruction of an Expression Vector Containing a Novel Fusion Sequence from Middle Region of NS3 and Truncated Core Genes of Hepatitis C Virus
Background and Aims: DNA constructs containing HCV antigens have become one of the vaccine candidates for induction of anti-HCV cellular and humoral immunity. In this study, we constructed a novel expressing vector harboring a fusion sequence derived from an overlapping fragment in the middle of NS3 and a truncated core fragment to avoid troubles reported to be associated with full gene express...
متن کاملDevelopment of Preventive Vaccines for Hepatitis C Virus E1/E2 Protein
Hepatitis C virus (HCV) is responsible for a vast majority of liver failure cases. HCV is a kind of blood disease appraised to chronically infect 3% of the worlds’ population causing significant morbidity and mortality. Therefore, a complete knowledge of humoral responses against HCV, resulting antibodies, and virus-receptor and virus-antibody interactions, are essential to design a vaccine. HC...
متن کاملCloning and expression of NS3 helicase fragment of hepatitis C virus and the study of its immunoreactivity in HCV infected patients
Objective(s): Hepatitis C is a major cause of liver failure worldwide. Current therapies applied for this disease are not fully effective and produce side effects in most cases. Non-structural protein 3 helicase (NS3) of HCV is one of the key enzymes in viral replication and infection. Therefore, this region is a promising target to design new drugs and therapies against HCV infection. The aim ...
متن کامل